Coding
Part:BBa_K4594006:Design
Designed by: Menghan Jia Group: iGEM23_ShanghaiTech-China (2023-10-01)
LuxB:cp157Venus
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1192
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1192
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1192
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1192
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Attention should be paid to the linker between LuxB and cp157Venus.
Source
Takeharu Nagai' s lab.